AI Article Synopsis
- The cgtA gene is crucial for the survival of Vibrio cholerae bacteria, even in strains lacking the relA gene.
- When CgtA levels were reduced, V. cholerae struggled to grow and became more vulnerable to the drug hydroxyurea.
- Overexpressing CgtA in E. coli led to the unusual elongation of the bacterial cells, with the C-terminal region of CgtA being vital for its function.
Article Abstract
The cgtA gene, coding for the conserved G protein CgtA, is essential in bacteria. In contrast to a previous report, here we show by using genetic analysis that cgtA is essential in Vibrio cholerae even in a Delta relA background. Depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. Overexpression of V. cholerae CgtA caused distinct elongation of Escherichia coli cells. Deletion analysis indicated that the C-terminal end of CgtA plays a critical role in its proper function.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446790 | PMC |
| http://dx.doi.org/10.1128/JB.02021-07 | DOI Listing |
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