The polytopic inner membrane protein MalF is a constituent of the MalFGK(2) maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK(2) maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1074/jbc.M801481200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Discovery of Novel ER-Localized Cellobiose Transporters Involved in Cellulase Biosynthesis in Trichoderma reesei.
J Basic Microbiol
December 2024
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Device, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Sugar transporters are of great importance in sensing and transporting varied sugars for cellulase biosynthesis of lignocellulolytic fungi. Nevertheless, the function and the relevant mechanism of sugar transporters in fungal cellulase biosynthesis remain to be explored. Here, putative maltose transporters Mal1, Mal2, Mal3, Mal4, and Mal5 in Trichoderma reesei were investigated.
View Article and Find Full Text PDFSqueeze pumping of lipids and insecticides by ABCH transporter.
Cell
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:
ATP-binding cassette (ABC) transporter subfamily H is only identified in arthropods and zebrafish. It transports lipids and is related to insecticide resistance. However, the precise mechanisms of its functions remain elusive.
View Article and Find Full Text PDFJianpi Huoxue Decoction Ameliorates Alcohol-associated Liver Disease by Improving Intestinal Barrier Function in Rats.
Curr Med Sci
December 2024
Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Objective: Jianpi huoxue decoction (JHD), a Chinese herbal formula, is commonly used for treating alcohol-associated liver disease (ALD). This study aimed to investigate the mechanism by which JHD affects intestinal barrier function in ALD rats.
Methods: The Sprague-Dawley rats were randomly divided into three groups: control group, model group and JHD group.
Specialization Restricts the Evolutionary Paths Available to Yeast Sugar Transporters.
Mol Biol Evol
November 2024
Laboratory of Genetics, J. F. Crow Institute for the Study of Evolution, Center for Genomic Science Innovation, DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI 53726, USA.
Article Synopsis
- Functional innovation at the protein level plays a significant role in evolution, with specific constraints depending on each protein's unique history and structure.
- The study focuses on a recent functional innovation in an α-glucoside transporter from the yeast Saccharomyces eubayanus, revealing that novel substrate transport requires complex interactions among various protein regions.
- By analyzing genome data from 332 Saccharomycotina yeast species, the research suggests that these α-glucoside transporters evolved from a multifunctional ancestor and underwent subfunctionalization, making the acquisition of new functions challenging but possible through specific genetic changes.
Genomic Characterization and Establishment of a Genetic Manipulation System for sp. ( Clade) LZ117.
J Fungi (Basel)
October 2024
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Article Synopsis
- Researchers found that a new strain called LZ117 is highly effective at producing enzymes that break down plant biomass and can also help control pests and plant diseases.
- They used DNA analysis to confirm that LZ117 is a unique species related to others in its group, and characterized its genetic makeup, including genes linked to enzyme production and transport mechanisms.
- The study showed that genetic manipulation of LZ117 is possible, including overexpressing a transporter gene, which does not hinder enzyme production but highlights the role of a specific gene in regulating enzyme production under glucose conditions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!