We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329591 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0002061 | PLOS |
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