A porphyrin-based nanopore was constructed through a bilayer lipid membrane (BLM). The macrocyclic porphyrin (the inner diameter = ca. 2.1 nm) having six carboxylic acid groups directed up and down was synthesized by self-assembly of three trisporphyrins, metathesis linking, and subsequent hydrolysis. The porphyrin was incorporated into soybean lecithin based BLM, and the ion currents through the nanopore were observed under 500 mM KCl, LiCl, CaCl2, and tetraalkylammonium chloride solutions, indicating formation of a large pore through BLM. Blocking of the ion current was achieved by addition of the polycationic fourth-generation PAMAM dendrimer.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja801129a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sequence-Dependent Slowdown of DNA Translocation Using Transmembrane RNA-DNA Interactions in MoS Nanopore.
J Phys Chem B
January 2025
Institute of Quantitative Biology, College of Life Sciences, and School of Physics, Zhejiang University, Hangzhou, Zhejiang 310058, China.
The emergence of nanopores in two-dimensional (2D) nanomaterials offers an attractive solid-state platform for high-throughput and low-cost DNA sequencing. However, several challenges remain to be addressed before their wide application, including the too-fast DNA translocation speed (compared to state-of-the-art single nucleoside detection techniques) and too large noise/signal ratios due to DNA fluctuations inside the nanopores. Here, we use molecular dynamics (MD) simulations to demonstrate the feasibility of utilizing RNA-DNA interactions in modulating DNA translocations in 2D MoS nanopores.
View Article and Find Full Text PDFEngineered sulfonated porous carbon/cellulose nanofiber hybrid membrane for high-efficiency osmotic energy conversion applications.
Int J Biol Macromol
January 2025
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China. Electronic address:
Harnessing ionic gradients to generate electricity has inspired the development of nanofluidic membranes with charged nanochannels for osmotic energy conversion. However, achieving high-performance osmotic energy output remains elusive due to the trade-off between ion selectivity and nanochannel membrane permeability. In this study, we report a homogeneous nanofluidic membrane, composed of sulfonated nanoporous carbon (SPC) and TEMPO-oxidized cellulose nanofibers (T-CNF), engineered to overcome these limitations.
View Article and Find Full Text PDFComparative Analysis of Active LTR Retrotransposons in Sunflower ( L.): From Extrachromosomal Circular DNA Detection to Protein Structure Prediction.
Int J Mol Sci
December 2024
All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya Str. 42, 127550 Moscow, Russia.
Plant genomes possess numerous transposable element (TE) insertions that have occurred during evolution. Most TEs are silenced or diverged; therefore, they lose their ability to encode proteins and are transposed in the genome. Knowledge of active plant TEs and TE-encoded proteins essential for transposition and evasion of plant cell transposon silencing mechanisms remains limited.
View Article and Find Full Text PDFSARS-CoV-2 Genomic Variants and Their Relationship with the Expressional and Genomic Profile of Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2).
Microorganisms
November 2024
Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Florianópolis, Florianópolis 88040-900, Santa Catarina, Brazil.
Over the past four years, angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) have been extensively studied, given their important role in SARS-CoV-2 replication; however, most studies have failed to compare their behavior in the face of different SARS-CoV-2 genomic variants. Therefore, this study evaluated the influence of different variants in ACE2/TMPRSS2 expressional and genomic profiles. To achieve this, 160 nasopharyngeal samples, previously detected with SARS-CoV-2 via RT-qPCR (June 2020-July 2022), were quantified for ACE2/TMPRSS2 expression levels, also using RT-qPCR; SARS-CoV-2 genomic variants, along with polymorphisms in the ACE2/TMPRSS2 coding genes, were identified using nanopore sequencing.
View Article and Find Full Text PDFHigh Proton Conductivity of Acid Impregnated COFs Stabilized by Post-Oxidation.
Small
November 2024
Department of Chemistry, Jilin University, Changchun, 130012, P. R. China.
Article Synopsis
- * Researchers improve the stability of an imine-based covalent organic framework (CPOF-10) by converting it to a more stable amide-linked version (CPOF-11), which allows for better integration of liquid proton-conducting acids.
- * The findings show that CPOF-11 exhibits significantly enhanced proton conductivity compared to its precursor, achieving one of the lowest activation energy values reported for proton-conducting materials, indicating potential applications in advanced porous organic materials for fuel
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!