We developed a novel in situ method for the control of F-actin assembly by using a synthetic photoresponsive polycation. The photoresponsive polycation mainly comprises a water-soluble cationic monomer and also contains a small amount of the monomer of a triphenylmethane leucohydroxide derivative (20 mol %), which is a well-known photochromic molecule that can be cationized in aqueous solution by ultra violet (UV) irradiation, thereby causing an increase in the total charge on the photoresponsive polycation. Thus, by exposure to UV radiation in aqueous solution, F-actin and the photoresponsive polycation start assembling into F-actin/photoresponsive polycation complexes of various morphologies such as bundles, coils, and networks, depending upon the concentrations of both the F-actin and salt. Further, localized UV irradiation can be applied in order to control the local formation of F-actin/photoresponsive polycation complexes. Thus, this technique provides a novel method for the spatiotemporal control of F-actin assembly and can be applied to investigate the unknown characteristics of F-actin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.31990 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Photoresponsive endosomal escape enhances gene delivery using liposome-polycation-DNA (LPD) nanovectors.
J Mater Chem B
August 2018
ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Department of Physics and Astronomy, Macquarie University, Sydney, New South Wales 2109, Australia.
Lipid-based nanocarriers with stimuli responsiveness have been utilized as controlled release systems for gene/drug delivery applications. In our work, by taking advantage of the high complexation capability of polycations and the light triggered properties, we designed a novel photoresponsive liposome-polycation-DNA (LPD) platform. This LPD carrier incorporates verteporfin (VP) in lipid bilayers and the complex of polyethylenimine (PEI)/plasmid DNA (pDNA) encoding EGFP (polyplex) in the central cavities of the liposomes.
View Article and Find Full Text PDFPhoto-responsive hollow silica nanoparticles for light-triggered genetic and photodynamic synergistic therapy.
Acta Biomater
August 2018
The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China. Electronic address:
Unlabelled: The development of multifunctional carriers incorporating genetic and photodynamic therapy (PDT) for synergistic antitumor treatment has attracted intensive interests very recently. However, most of the currently reported systems employ passive gene release strategies depending on tumor microenvironment, which are negatively affected by the heterogeneity of cancer cells, thus resulting in limited controllability in therapeutic progress. Herein, a novel photo-responsive hollow silica nanoparticle (HNP)-based gene and photosensitizer (PS) co-delivery nanovehicle is designed for dual-wavelength light-triggered synergistic gene and PDT therapy.
View Article and Find Full Text PDFSupramolecular host-guest polycationic gene delivery system based on poly(cyclodextrin) and azobenzene-terminated polycations.
Colloids Surf B Biointerfaces
November 2016
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China. Electronic address:
This article describes the supramolecular host-guest polycationic gene delivery system based on poly(β-cyclodextrin) (PCD) and azobenzene-terminated polycations. The azobenzene-terminated linear (Az-LPDM) and branched (Az-BPDM) cationic polymers were synthesized by atom transfer radical polymerization (ATRP) of 2-dimethylamino ethyl methacrylate (DMAEMA). The formation and photosensitive behavior of the supramolecular polycations of azobenzene-terminated polycations Az-LPDM and Az-BPDM with PCD were confirmed by UV-vis and NMR analysis.
View Article and Find Full Text PDFUV light-triggered unpacking of DNA to enhance gene transfection of azobenzene-containing polycations.
J Mater Chem B
June 2014
School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, PR China.
This study demonstrates a strategy to enhance gene delivery via photoregulated gene unpacking from its vector. Photoresponsive polycationic vectors composed of a middle azobenzene moiety and two terminal blocks of poly[2-(dimethylamino)ethyl methacrylate], termed as Azo-PDMAEMA, are synthesized using a difunctional azobenzene-based initiator via atomic transfer radiator polymerization (ATRP). The Azo-PDMAEMA exhibits trans to cis isomerization under alternate Vis-UV irradiation, and is capable of condensing plasmid DNA into nanocomplexes.
View Article and Find Full Text PDFVisible-light-induced disruption of diselenide-containing layer-by-layer films: toward combination of chemotherapy and photodynamic therapy.
Small
December 2013
Key Laboratory of Organic Optoelectronics & Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
A photoresponsive polyelectrolyte multilayer film containing a diselenide functional group is fabricated using an unconventional layer-by-layer method. The polycation backbone is constructed through copolymerization of di-(1-hydroxylundecyl) diselenide and 1,4-bis(2-hydroxyethyl)piperazine with 2,4-diisocyanatotoluene. A common polyanion poly(styrene sulfonate) is selected as the polyanion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!