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Techniques for mammalian cell tissue culture. | LitMetric

Techniques for mammalian cell tissue culture.

Curr Protoc Protein Sci

Molecular Pathology Laboratory Network, Maryville, Tennessee.

Published: December 2006

This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

Download full-text PDF

Source
http://dx.doi.org/10.1002/0471140864.psa03cs46DOI Listing

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