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Synthesis and characterization of chimeric 2-5A-DNA oligonucleotides. | LitMetric

Synthesis and characterization of chimeric 2-5A-DNA oligonucleotides.

Curr Protoc Nucleic Acid Chem

Northern Arizona University, Flagstaff, Arizona, USA.

Published: May 2001

This unit provides protocols for the synthesis and characterization of 2-5A-antisense nucleic acids. These chimeric oligonucleotides consist of 2',5'-phosphodiester-linked oligoadenylates ligated to 3',5'-deoxyribonucleotides and are readily prepared using phosphoramidite chemistry on CPG solid supports. The 3',5'-deoxyribonucleotide functions as the antisense domain to target a given mRNA sequence, while the 2',5'-phosphodiester-linked oligoadenylate serves to locally activate 2-5A-dependent RNase L, causing the targeted sequence to be cleaved.

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Source
http://dx.doi.org/10.1002/0471142700.nc0404s01DOI Listing

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