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Construction of replication-defective herpes simplex virus vectors. | LitMetric

AI Article Synopsis

  • * Herpes simplex virus (HSV) vectors are effective for delivering genes to neurons in the central and peripheral nervous systems.
  • * The text outlines methods for creating cell lines to support mutants, generating replication-defective virus variants, and inserting foreign DNA into these viral genomes as part of vector preparation, along with protocols for working with virus stocks and DNA.

Article Abstract

Advances in identification and characterization of gene products responsible for specific diseases of the nervous system have opened opportunities for novel therapies using gene transfer vectors for gene replacement. Herpes simplex virus (HSV)-based vectors are particularly well suited for gene delivery to neurons of the central and peripheral nervous systems. The authors have developed methods to delete HSV-1 IE gene functions and to subsequently introduce foreign genes into the HSV-1 genome using homologous recombination. This unit describes methods for generating cell lines that complement multiple essential gene deletion mutants as well for generating such replication-defective virus recombinants and inserting foreign DNA sequences into replication-defective viral genomes, the last step in preparing a vector. Three support protocols describe methods for preparing virus stocks, titering virus, and preparing viral DNA.

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Source
http://dx.doi.org/10.1002/0471142905.hg1211s33DOI Listing

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