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Measurement of protein S-nitrosylation during cell signaling. | LitMetric

Measurement of protein S-nitrosylation during cell signaling.

Methods Enzymol

Department of Medicine and Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Published: June 2008

AI Article Synopsis

Article Abstract

S-Nitrosylation, the modification of a cysteine thiol by a nitric oxide (NO) group, has emerged as an important posttranslational modification of signaling proteins. An impediment to studying the regulation of cell signaling by S-nitrosylation has been the technical challenge of detecting endogenously S-nitrosylated proteins. Detection of S-nitrosylated proteins is difficult because the S-NO bond is labile and therefore can be lost or gained artifactually during sample preparation. Nevertheless, several methods have been developed to measure endogenous protein S-nitrosylation, including the biotin switch assay and the chemical reduction/chemiluminescence assay. This chapter describes these two methods and provides examples of how they have been used successfully to elucidate the role of protein S-nitrosylation in cell physiology and pathophysiology.

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Source
http://dx.doi.org/10.1016/S0076-6879(07)00814-2DOI Listing

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