Objective: To probe into feasibility of extraction techniques for Vernonia anthelmintica using HPLC fingerprint of active part as model.
Methods: The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices.
Results: The optimum extracting procedure was as follows: using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each.
Conclusion: It is scientific, simple and applicable.
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