An improved version of the three-dimensional HCCH-COSY NMR experiment is described that correlates the resonances of geminal and vicinal proton pairs with the chemical shift of the 13C nucleus attached to one of the protons. The experiment uses constant-time evolution of transverse 13C magnetization which optimizes transfer of magnetization and thus improves the sensitivity of the experiment over the original scheme. The experiment is demonstrated for calmodulin complexed with a 26-residue peptide comprising the binding site of skeletal muscle myosin light chain kinase.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF01875522 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fixation probability in evolutionary dynamics on switching temporal networks.
J Math Biol
September 2023
Department of Mathematics, State University of New York at Buffalo, Buffalo, NY, 14260-2900, USA.
Population structure has been known to substantially affect evolutionary dynamics. Networks that promote the spreading of fitter mutants are called amplifiers of selection, and those that suppress the spreading of fitter mutants are called suppressors of selection. Research in the past two decades has found various families of amplifiers while suppressors still remain somewhat elusive.
View Article and Find Full Text PDFEvolution of confined quantum scalar fields in curved spacetime. Part II: Spacetimes with moving boundaries in any synchronous gauge.
Eur Phys J C Part Fields
October 2021
Quantenoptik, Quantennanophysik und Quanteninformation, Fakultät für Physik, Universität Wien, Boltzmanngasse 5, 1090 Vienna, Austria.
We develop a method for computing the Bogoliubov transformation experienced by a confined quantum scalar field in a globally hyperbolic spacetime, due to the changes in the geometry and/or the confining boundaries. The method constructs a basis of solutions to the Klein-Gordon equation associated to each compact Cauchy hypersurface of constant time. It then provides a differential equation for the linear transformation between bases at different times.
View Article and Find Full Text PDFDetecting Lineage-Specific Shifts in Diversification: A Proper Likelihood Approach.
Syst Biol
February 2021
Groningen Institute for Evolutionary Life Sciences, University of Groningen, Box 11103, 9700 CC, Groningen, The Netherlands.
The branching patterns of molecular phylogenies are generally assumed to contain information on rates of the underlying speciation and extinction processes. Simple birth-death models with constant, time-varying, or diversity-dependent rates have been invoked to explain these patterns. They have one assumption in common: all lineages have the same set of diversification rates at a given point in time.
View Article and Find Full Text PDFMirror symmetric broadband homodecoupled perfect echo spectroscopy.
J Magn Reson
June 2020
Bruker BioSpin GmbH, Silberstreifen, D-76287 Rheinstetten, Germany. Electronic address:
A new experiment for recording phase sensitive ω-broadband homodecoupled TOCSY spectra is presented. The method is an extension of the already existing perfect echo (PE) filter, proposed to sample t chemical shift under sustained homodecoupling. The modification is made by attaching a time reversed perfect echo filter to a regular perfect echo scheme.
View Article and Find Full Text PDFImproved sensitivity of laser-enhanced H-C-correlation via suppression of C-C' scalar-coupling evolution.
J Magn Reson
October 2019
Department of Chemistry, University of Wisconsin - Madison, 1101 University Ave., Madison, WI 53706, USA. Electronic address:
Low-concentration photochemically induced dynamic polarization (LC-photo-CIDNP) enables the spectroscopic analysis of biomolecules containing the amino acids Trp and Tyr at sub-micromolar concentration in solution. Typical LC-photo-CIDNP pulse sequences involving H-C correlation, however, perform well in the case of aromatic resonances but display a relatively poor signal-to-noise ratio for C and C resonances. Here, we develop a novel pulse sequence denoted as C perturbation-recovered selective-pulse photo-CINDP enhanced reverse INEPT, or C PRESPRINT, tailored to the LC-photo-CIDNP analysis of H-C pairs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!