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Isolation of Natural Products Using Preparative TLC.
Methods Mol Biol
January 2025
Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
Known for their diverse and potent physiological activities, natural products continue to be essential for the discovery and development of new drugs. This chapter explores the pivotal role of preparative thin-layer chromatography (Prep-TLC) in the isolation of natural products. This chapter begins with an understanding of the historical significance and structural complexity of natural products, and discusses the problems caused by complex mixtures present in extracts, as well as the multifunctionality, cost-effectiveness, and compatibility with different sample types of Prep-TLC to address these challenges.
View Article and Find Full Text PDFIdentification of Lignans.
Methods Mol Biol
January 2025
College of Pharmacy and Institute of Pharmaceutical Sciences and Technology, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
Lignans have garnered significant interest in various fields of pharmaceuticals, nutrition, and pesticides due to their diverse biological activities. This chapter primarily focuses on the extraction and purification methods of lignans from Schisandra, followed by an extensive examination of qualitative and quantitative analytical techniques, including thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Additionally, methods for structural analysis using nuclear magnetic resonance (NMR) are briefly summarized.
View Article and Find Full Text PDFIsolation and Identification of Coumarins from Citrus latifolia Leaves Using Advanced Chromatographic Techniques.
Methods Mol Biol
January 2025
Laborotorio de Química de Products Naturales, Departmento de Biotecnologia y Bioquímica, Centro de Investigatión y de Estudios Avanzados de IPN, Guanajuato, Mexico.
Coumarins are a class of natural compounds that exhibit a wide range of biological activities, which include anticoagulant, anti-inflammatory, anticancer, and antimicrobial effects. This class of compounds has drawn considerable interest in recent years. The exploration of such properties contributes to our understanding of the potential of coumarins as valuable agents in various clinical applications.
View Article and Find Full Text PDFIdentification of Flavonoids Using UV-Vis and MS Spectra.
Methods Mol Biol
January 2025
Plant Sciences, Institute of Biology, Leiden University, Leiden, The Netherlands.
Flavonoids are a group of specialized metabolites that are ubiquitously found within the plant kingdom. While they fulfill various important functions within the plant, they are also utilized by humans in a variety of different fields such as medicine, food science, and agriculture. Thus, to elucidate the chemical composition of any given plant extract, extraction and identification of flavonoids are of high interest.
View Article and Find Full Text PDFStandardized HPTLC for Reproducible Chemical Fingerprinting.
Methods Mol Biol
January 2025
HPTLC Association, Rheinfelden, Switzerland.
High-performance thin-layer chromatography (HPTLC) plays a crucial role in establishing the chemical fingerprint of natural products (NPs) during analysis. This technique involves standardized procedures for each step, ensuring the reproducibility of results. In this context, we present a comprehensive standard operating procedure (SOP) encompassing both manual instruments and suitable devices.
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