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| http://dx.doi.org/10.1093/nar/19.17.4787 | DOI Listing |
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Dynamic interactions of retroviral Gag condensates with nascent viral RNA at transcriptional burst sites: implications for genomic RNA packaging.
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Department of Medicine, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033.
Retroviruses are responsible for significant pathology in humans and animals, including the acquired immunodeficiency syndrome and a wide range of malignancies. A crucial yet poorly understood step in the replication cycle is the recognition and selection of unspliced viral RNA (USvRNA) by the retroviral Gag protein, which binds to the psi (Ψ) packaging sequence in the 5' leader, to package it as genomic RNA (gRNA) into nascent virions. It was previously thought that Gag initially bound gRNA in the cytoplasm.
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Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA
The cell adhesion molecule Leucine-Rich Repeat Transmembrane neuronal protein 2 (LRRTM2) is crucial for synapse development and function. However, our understanding of its endogenous trafficking has been limited due to difficulties in manipulating its coding sequence (CDS) using standard genome editing techniques. Instead, we replaced the entire LRRTM2 CDS by adapting a two-guide CRISPR knock-in method, enabling complete control of LRRTM2.
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Forage & Range Research Laboratory, USDA-ARS, Logan, UT 84322-6300, USA.
Soil salinity adversely affects plant growth and development, reducing the yield of most crops, including wheat. The highly salt-tolerant wheat germplasm lines W4909 and W4910 were derived from a cross between two moderately salt-tolerant lines, the Chinese Spring (CS)/ disomic addition line AJDAj5 (AJ) and the Ph-inhibitor line (Ph-I) derived from CS/. Molecular markers for gene introgressions in W4909 and W4910 were not reported.
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Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Infection by human astrovirus (HAstV), a small, positive-strand RNA virus, is a major cause of gastroenteritis and has been implicated in an increasing number of severe, sometimes fatal, neurological diseases since 2008. Currently, there are no vaccines or antiviral treatments available to treat HAstV infection. An attractive target for antiviral therapeutics is the viral protease due to its essential functions throughout infection.
View Article and Find Full Text PDFEndo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources.
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Regensburg Center for Biochemistry (RCB), Laboratory for RNA Biology, University of Regensburg, Regensburg, Germany
Article Synopsis
- RNA binding proteins (RBPs) are essential for gene regulation by recognizing specific RNA targets, and various methods exist to study their binding preferences, like CLIP and SELEX.
- Current methods face challenges such as the need for purified proteins and extensive sequencing, which can limit their usage.
- The proposed RNA bind-n-seq method simplifies the process by using random RNA pools and allows for multiple selection rounds, improving motif identification without needing recombinant proteins.
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