AI Article Synopsis
- The study aimed to assess changes in histone modifications in patients with systemic lupus erythematosus (SLE) by analyzing CD4+ T cells.
- Significant hypoacetylation of histones H3 and H4 was found in active lupus patients compared to healthy controls, correlating with increased disease activity.
- Additionally, there was hypomethylation of histone H3K9 observed in both active and inactive SLE, while certain chromatin modifier genes showed altered mRNA levels in active cases, indicating abnormal histone modifications in SLE.
Article Abstract
Objective: To investigate alterations in histone modifications in patients with systemic lupus erythematosus (SLE).
Methods: Global histone H3/H4 acetylation and H3K4/H3K9 methylation in CD4+ T cells from 20 SLE patients and 10 healthy control subjects were assayed using the EpiQuik global histone H3/H4 acetylation and H3K4/H3K9 methylation assay kits. mRNA levels of 12 members of 3 classes of chromatin modifier genes were measured by real-time quantitative polymerase chain reaction.
Results: Global histone H3 and H4 hypoacetylation was observed in active lupus CD4+ T cells compared with controls (p = 0.002 and p = 0.009, respectively). The degree of histone H3 acetylation correlated negatively with increased disease activity in lupus patients as measured by SLEDAI (r = -0.889, p = 0.044). We found global histone H3K9 hypomethylation in both active and inactive lupus CD4+ T cells, compared with controls (p = 0.001, p = 0.003, respectively). However, global levels of H3K4 methylation were not different between patients and controls. SIRT1 mRNA levels were significantly increased in active lupus CD4+ T cells compared with controls (p < 0.001), while mRNA levels of CREBBP, P300, HDAC2, HDAC7, SUV39H2, and EZH2 were significantly downregulated in patients with active lupus (p < 0.001, p < 0.001, p = 0.01, p < 0.001, p = 0.003, p = 0.001, respectively).
Conclusion: Histone modifications appear abnormal in CD4+ T cells in SLE.
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