The objective of this study was to improve the accumulation of ginsenosides by the adventitious root cultures of ginseng, which are important secondary metabolites with pharmaceutical applications. The adventitious roots were cultured in bioreactors for 50 d using 1.5-strength Murashige and Skoog (MS) medium supplemented with 10 mg/l indole acetic acid and 75 g/l sucrose. Kinetic studies of the nutrient composition of the spent medium revealed the gradual depletion of various inorganic nutrients and sugars. Cultures were supplied with fresh nutrient medium (medium exchange or replenishment with 0.75- and 1.0-strength MS medium) after 10 and 20 d of culture initiation to fulfill the nutritional requirements of adventitious roots. Medium replenishment strategy (with 1.0-strength MS medium after 20 d of culture) significantly improved the growth of adventitious roots and the biosynthesis of ginsenosides by the adventitious roots. This work is useful for the large-scale cultivation of adventitious roots for the production of ginsenosides.
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http://dx.doi.org/10.1263/jbb.105.288 | DOI Listing |
Front Plant Sci
December 2024
Leibniz University Hannover, Institute of Horticultural Production Systems, Section Woody Plant and Propagation Physiology, Hannover, Germany.
Introduction: The presence of wounds in addition to the excision-induced wounds after severance from the stock plants is known to positively influence adventitious root formation of woody plant cuttings. Previous morphological studies highlighted laser wounding as a technique allowing to precisely control the decisive ablation depth. However, the biochemical processes involved in the response of rooting to the additional wounding remained unexplored.
View Article and Find Full Text PDFBur., a versatile plant with medicinal, edible, landscaping, and ecological applications, holds significant economic value and boasts a long-standing history of utilization in China. Despite its robust adaptability, rapid growth, and extensive distribution, the current research gap concerning the physiological mechanisms underlying stem cutting propagation hampers the development of efficient strategies for commercial-scale propagation of , particularly for large-scale cultivation.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
Tea Research Institute, Anhui Academy of Agricultural Sciences, Huangshan, 245000, China.
Background: Adventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this process.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
The Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.
Background: WRKY transcription factors are plant-specific and play essential roles in growth, development, and stress responses, including reactions to salt, drought, and cold. Despite their significance, the WRKY genes in the wild sweet potato ancestor, Ipomoea pes-caprae, remain unexplored.
Results: In this study, 65 WRKY genes were identified in the I.
BMC Plant Biol
December 2024
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing, Jiangsu, 210037, P.R. China.
Cinnamomum parthenoxylon is a significant essential oil plant in southern China, however, the challenge of rooting cuttings poses a hindrance to its development and widespread cultivation. Adventitious root (AR) formation is a vital mechanism for plants to acclimate to environmental changes, yet the precise regulatory mechanisms governing this process remain largely unknown. This study investigated the morphological, physiological, and transcriptomic alterations during AR formation in C.
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