Retrospective analysis of monkeypox infection.

Emerg Infect Dis

Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA.

Published: April 2008

Serologic cross-reactivity between orthopoxviruses is a substantial barrier to laboratory diagnosis of specific orthopoxvirus infections and epidemiologic characterization of disease outbreaks. Historically, time-consuming and labor-intensive strategies such as cross-adsorbed neutralization assays, immunofluorescence assays, and hemagglutination-inhibition assays have been used to identify orthopoxvirus infections. We used cross-adsorption to develop a simple and quantitative postadsorption ELISA for distinguishing between monkeypox and vaccinia infections. Despite the difficulty of diagnosing clinically inapparent monkeypox in previously vaccinated persons, this technique exhibited 100% sensitivity and 100% specificity for identifying clinically overt monkeypox infection irrespective of vaccination history. We also describe a Western blot technique in which up to 3 diagnostic bands may be used to distinguish between vaccinia and monkeypox infection. The techniques described provide independent diagnostic tests suitable for retrospective analysis of monkeypox outbreaks.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570942PMC
http://dx.doi.org/10.3201/eid1404.071044DOI Listing

Publication Analysis

Top Keywords

monkeypox infection
12
retrospective analysis
8
analysis monkeypox
8
orthopoxvirus infections
8
monkeypox
6
infection serologic
4
serologic cross-reactivity
4
cross-reactivity orthopoxviruses
4
orthopoxviruses substantial
4
substantial barrier
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!