AI Article Synopsis
- * Using the polymerase chain reaction (PCR) method, DNA samples were taken from 100 unrelated individuals, and the results showed no deviations from Hardy-Weinberg equilibrium, indicating a stable genetic structure.
- * The loci exhibited high expected heterozygosities and discriminating powers, suggesting that the last four loci are effective for individual identification and paternity testing in forensic science.
Article Abstract
Objective: To get the preliminary genotype and allele frequency distributions of GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 loci in Chinese Han population in Chengdu area, and to validate more short tandem repeat (STR) systems for forensic application.
Methods: The polymerase chain reaction (PCR) was used to this study. Five STRs (GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05) were amplified from DNA samples, which were extracted with Chelex-100 method from EDTA-blood of 100 unrelated individuals. The PCR products were analyzed by PAG vertical electrophoresis.
Results: No deviations from Hardy-Weinberg equilibrium were observed. The expected heterozygosities observed were 0.694, 0.918, 0.836, 0.889 and 0.880 for GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 respectively. The discriminating powers were 0.697, 0.901, 0.875, 0.900 and 0.891.
Conclusion: The last four loci in this study are useful markers for genetics purposes in individual identification and paternity test.
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