Flow cytometric analysis of canine umbilical cord blood lymphocytes.

J Vet Med Sci

Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.

Published: March 2008

AI Article Synopsis

  • * In umbilical cord blood, the proportions were 52.9% CD3+ T lymphocytes, 30.4% CD21+CD3- B lymphocytes, and 16.7% CD3-CD21- non-T non-B lymphocytes.
  • * The T/B lymphocyte ratio was significantly lower in umbilical cord blood, while the CD4+/CD8+ lymphocyte ratio was significantly higher compared to the peripheral blood of the dams.

Article Abstract

Lymphocyte subsets in canine umbilical cord blood were flow cytometrically analyzed and compared with those of the dams' peripheral blood. The proportion of CD3+ T lymphocytes, CD21+CD3- B lymphocytes, and CD3-CD21- non-T non-B lymphocytes in umbilical cord blood was 52.9%, 30.4%, and 16.7%, respectively. T lymphocyte/B lymphocyte ratio was significantly lower in the umbilical cord blood than in the dams' peripheral blood (2.1 +/- 1.4 versus 11.0 +/- 8.1, P < 0.001). In contrast, CD4+ lymphocyte/CD8+ lymphocyte ratio was significantly higher in the umbilical cord blood than in the dams' peripheral blood (7.6 +/- 2.2 versus 1.8 +/- 0.6, P<0.001). These findings clarified the phenotypic characters of canine umbilical cord blood lymphocytes.

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Source
http://dx.doi.org/10.1292/jvms.70.285DOI Listing

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