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Dual fluorescent molecular substrates selectively report the activation, sustainability and reversibility of cellular PKB/Akt activity.
Sci Rep
October 2013
Department of Radiology, Washington University in St. Louis, Missouri 63110, USA.
Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme's activity in real time. Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. The NIR DCF probe was highly selective for the Akt1 isoform, which was demonstrated using Akt1 knockout cells derived from MMTV-ErbB2 transgenic mice.
View Article and Find Full Text PDFSynthesis and spectroscopy of near infrared fluorescent dyes for investigating dichromic fluorescence.
Bioorg Med Chem Lett
January 2011
Department of Radiology, Washington University, 4525 Scott Avenue, St Louis, MO 63110, United States.
We developed a series of near infrared (NIR) cyanine dyes to study dichromic fluorescence phenomenon, which provides new protocols for in vivo optical imaging. Preliminary spectroscopic studies show that dichromic fluorescence correlates with structural symmetry. This feature suggests the potential use of dichromic fluorescent molecules to study biological processes that can alter the structural symmetry of the molecular probes.
View Article and Find Full Text PDFUnderstanding Dichromic Fluorescence Manifested in Certain ICG Analogs.
Pure Appl Chem
January 2010
Departments of Radiology, Washington University, St. Louis, Missouri 63110, USA.
Fluorescence has advanced our understanding in various aspects of biological processes. Fluorescence in the near infrared (NIR) region avoids background autofluorescence from biological samples leading to improved image quality. In searching for indocyanine green (ICG) analogs that can be attached to biomolecules, we observed that dichromic fluorescence manifested in some mono reactive-group functionalized ICG analogs.
View Article and Find Full Text PDFNear-infrared dichromic fluorescent carbocyanine molecules.
Angew Chem Int Ed Engl
May 2008
Department of Radiology, Washington University School of Medicine, 4525 Scott Avenue, St. Louis, MO 63110, USA.
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