Antifeeding properties of myosuppressin in a generalist phytophagous leafworm, Spodoptera littoralis (Boisduval).

Regul Pept

Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona (CSIC), Jordi Girona, 18-26, 08034 Barcelona, Spain.

Published: June 2008

Insect myosuppressins are a family of peptides with a characteristic HV/SFLRFamide carboxy terminus. They are expressed in brain, neurohemal organs, stomatogastric nervous system, and in midgut endocrine cells. From a functional point of view, myosuppressins inhibit contractions of different visceral muscles, stimulate certain skeletal muscles and activate enzyme secretion from the gut. Moreover, in the omnivorous cockroach Blattella germanica, myosuppressin inhibits food intake. Based on these results, we studied the antifeeding activity of myosuppressin in the phytophagous leafworm Spodoptera littoralis. Firstly, we isolated the cDNA corresponding to the S. littoralis myosuppressin precursor encoding the typical myosuppressin peptide of lepidopterans: pQDVVHSFLRFamide. Then, we determined the expression patterns (in terms of mRNA and peptide) of myosuppressin in brain and midgut, and peptide levels in the haemolymph. Myosuppressin patterns in the brain and haemolymph were similar, and symmetrical to that of food consumption, thus suggesting that myosuppressin might inhibit feeding in S. littoralis. Moreover, synthetic myosuppressin effectively inhibited food intake in non-choice antifeeding tests. Taken together, the obtained results point to the hypothesis that myosuppressin represses feeding in S. littoralis.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.regpep.2008.02.002DOI Listing

Publication Analysis

Top Keywords

myosuppressin
10
phytophagous leafworm
8
leafworm spodoptera
8
spodoptera littoralis
8
food intake
8
feeding littoralis
8
littoralis
5
antifeeding properties
4
properties myosuppressin
4
myosuppressin generalist
4

Similar Publications

Myosuppressin signaling deficiency affects ovarian development via repression of 20-hydroxyecdysone biosynthesis in Grapholita molesta.

Pestic Biochem Physiol

November 2024

College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China; Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030031, Shanxi, China. Electronic address:

The steroid 20-hydroxyecdysone (20E) is crucial in regulating ovarian development. However, the neuropeptidergic mechanisms underlying ovarian development via 20E are underexplored. In this study, we investigated myosuppressin (MS) signaling in the dominant fruit pest Grapholita molesta and revealed that MS signaling is necessary for 20E biosynthesis during ovarian maturation.

View Article and Find Full Text PDF

Molecular Functions and Physiological Roles of Gustatory Receptors of the Silkworm .

Int J Mol Sci

September 2024

Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, Naka 2-24-16, Koganei 184-8588, Tokyo, Japan.

Article Synopsis
  • * Gene knockout studies highlight the role of specific Grs, like BmGr6 and BmGr9, in identifying feeding-promoting compounds from mulberry leaves and suggest that these receptors contribute to host recognition without actually feeding.
  • * Insights gained from cryogenic electron microscopy have uncovered how the BmGr9 ion channel operates, showing that it opens in response to fructose, indicating novel signaling mechanisms distinct from traditional G protein pathways.
View Article and Find Full Text PDF

To regulate physiological homeostasis and behavior in Bombyx mori, more than 20 peptide hormones in the midgut of larvae are secreted upon detection of food substances at the lumen. Although it is logical to assume that the timings of peptide hormone secretions are regulated, little is known about the mechanisms. In this study, the distributions of enteroendocrine cells (EECs) producing five peptide hormones and EECs expressing gustatory receptors (Grs), as candidate receptors for luminal food substances and nutrients, were examined via immunostaining in B.

View Article and Find Full Text PDF

Neuropeptides are important regulators of animal physiology and behavior. Hitherto the gold standard for the localization of neuropeptides have been immunohistochemical methods that require the synthesis of antibody panels, while another limiting factor has been the brain's opacity for subsequent in situ light or fluorescence microscopy. To address these limitations, we explored the integration of high-resolution mass spectrometry imaging (MSI) with microtomography for a multiplexed mapping of neuropeptides in two evolutionary distant ant species, and .

View Article and Find Full Text PDF

The gross anatomy of the male reproductive structures and their associated musculature are described in the blood-gorging vector of Chagas disease, . The male reproductive system is composed of muscular tissues each performing contractions that aid in the movement of sperm out of the testis into the vas deferens, seminal vesicle and then into the ejaculatory duct, along with proteins and lipids from the transparent and opaque accessory glands. Phalloidin staining shows the various patterns of muscle fiber layers, from thin circular to more complex crisscross patterns, implying subtle differences in the form of the contractions and movement of each of the structures, allowing for waves of contractions or twisting patterns.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!