A novel chemotaxonomical method based on 1D (1)H HRMAS NMR spectroscopy is being tested for taxonomical purposes. This powerful technique allowed us to discriminate between specimens belonging to two sister species of Turbinaria, which are difficult to tell apart using only morphological characters. Based on spectra analysis, the results allowed us to successfully group the specimens according to their species. Thus, the efficiency of HRMAS NMR spectroscopy for the discrimination of algal species and for the pre-screening of potential chemomarkers is demonstrated.
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http://dx.doi.org/10.1016/j.talanta.2007.08.021 | DOI Listing |
Methods Mol Biol
January 2025
NMR Laboratory, Chemistry Department, University of Crete, Heraklion, Crete, Greece.
High Resolution-Magic Angle Spinning (HR-MAS) solid-state NMR spectroscopy is finding increasing application in the analysis of solid foods, bypassing the need for complicated solvent extraction procedures. In the present protocol, we report a simple analytical approach based on HR-MAS NMR spectroscopy for the phenolic profiling of olive fruits, flesh, or skin. This approach allows the facile characterization of phenolic compounds in olive fruits cultivated for extra-virgin olive oil production as a function of maturation and variety, in addition to processing technology for table olives.
View Article and Find Full Text PDFGels
December 2024
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Químicas, Ciudad Autónoma de Buenos Aires 1113, Argentina.
In this work, the mechanical properties of hydrogels based on linear polyethyleneimine (PEI) chemically crosslinked with ethyleneglycoldiglycidyl ether (EGDE) were improved by the ionic crosslinking with sodium tripolyphosphate (TPP). To this end, the quaternization of the nitrogen atoms present in the PEI structure was conducted to render a network with a permanent positive charge to interact with the negative charges of TPP. The co-crosslinking process was studied by H high-resolution magic angle spinning (H HRMAS) NMR and X-ray photoelectron spectroscopy (XPS) in combination with organic elemental analysis and inductively coupled plasma mass spectrometry (ICP-MS).
View Article and Find Full Text PDFSkelet Muscle
December 2024
Department of Physiology and Aging, University of Florida, Gainesville, FL, USA.
Despite its notoriously mild phenotype, the dystrophin-deficient mdx mouse is the most common model of Duchenne muscular dystrophy (DMD). By mimicking a human DMD-associated metabolic comorbidity, hyperlipidemia, in mdx mice by inactivating the apolipoprotein E gene (mdx-ApoE) we previously reported severe myofiber damage exacerbation via histology with large fibro-fatty infiltrates and phenotype humanization with ambulation dysfunction when fed a cholesterol- and triglyceride-rich Western diet (mdx-ApoE). Herein, we performed comparative lipidomic and metabolomic analyses of muscle, liver and serum samples from mdx and mdx-ApoE mice using solution and high-resolution-magic angle spinning (HR-MAS) H-NMR spectroscopy.
View Article and Find Full Text PDFCarbohydr Polym
February 2025
Dipartimento di Chimica, Materiali e Ingegneria Chimica "G. Natta", Politecnico di Milano, via Mancinelli 7, I-20131 Milano, MI, Italy. Electronic address:
Anal Methods
January 2025
Council for Research in Agriculture and Agricultural Economy Analysis (CREA), Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.
Food quality is a crucial issue for producers and consumers, either dealing with commodities according to basic standards or with top quality products. Among the parameters contributing to quality, the place of origin is considered to be one of the most relevant, especially for protected denomination of origin and protected geographical indication foods, PDO and PGI, respectively. These labels have been designed by the EU to protect and valorise high quality foodstuff produced in limited areas and to ensure higher incomes to farmers.
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