The recombinant plasmids have been constructed encoding the synthesis of a full-sized diphtheria toxin from its own or PR, PL-promoters of bacteriophage lambda in Escherichia coli cells. The high level constitutive synthesis of toxin results in slow cell growth and plasmid elimination. The toxin was mainly detected in the periplasm, partially in the membrane and to a less extent in the cytoplasm and culturing medium. The dimeric form of toxin was found in the cytoplasm. Participation of toxin B-subunit in secreting of the toxin into culturing medium is discussed. Proteolytic degradation of the synthesized toxin in different Escherichia coli strains was demonstrated. The process takes place in cytoplasm and periplasm mainly. The main enzyme participating in the process is a La-protease. The data on proteolysis obtained by immunoprecipitation immunoblotting, affinity chromatography and in mini-cells of Escherichia coli are summarized.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A multifunctional photothermal electrospun PLGA/MoS@Pd nanofiber membrane for diabetic wound healing.
Regen Biomater
December 2024
Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
Injury caused by excess reactive oxygen species (ROS) may lead to susceptibility to bacterial infection and sustained inflammatory response, which are the major factors impeding diabetic wound healing. By utilizing optimal anti-inflammatory, antioxidant and antibacterial biomaterials for multifunctional wound dressings is critical in clinical applications. In this study, a novel electrospun PLGA/MoS@Pd nanofiber membrane was synthesized by encapsulating antioxidant and near-infrared (NIR) responsive MOS@Pd nanozymes in PLGA nanofibers to form a multifunctional dressing for diabetic wound repair.
View Article and Find Full Text PDFPhenotypic and genetic extended spectrum beta lactamase profiles of bacterial isolates from ICU in tertiary level hospital in Kenya.
F1000Res
January 2025
Department of Human Pathology, University of Nairobi, Nairobi, Nairobi County, Kenya.
Background: Bacterial infections in the Intensive Care Units are a threat to the lives of critically ill patients. Their vulnerable immunity predisposes them to developing bacteria-associated sepsis, deteriorating their already fragile health. In the face of increasing antibiotics resistance, the problem of bacterial infection in ICU is worsening.
View Article and Find Full Text PDFDynamic association of antimicrobial resistance in urinary isolates of and between primary care and hospital settings in the Netherlands (2008-2020): a population-based study.
Lancet Reg Health Eur
March 2025
Department of Biostatistics, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands.
Background: It is unclear whether changes in antimicrobial resistance (AMR) in primary care influence AMR in hospital settings. Therefore, we investigated the dynamic association of AMR between primary care and hospitals.
Methods: We studied resistance percentages of and isolates to co-amoxiclav, ciprofloxacin, fosfomycin, nitrofurantoin and trimethoprim submitted by primary care, hospital outpatient and hospital inpatient settings to the Dutch National AMR surveillance network (ISIS-AR) from 2008 to 2020.
A Cross-Sectional Study of the Maternal Health Factors: The Interplay Between Breastfeeding Patterns, Gut Microbiota, Anemia, and Cardiovascular Risk in Lactating Mothers.
Cureus
December 2024
Medicine, Army Medical College, Rawalpindi, PAK.
Objective This cross-sectional study explored the interplay between breastfeeding patterns, gut microbiota composition, anemia, and cardiovascular risk in lactating mothers. The study examined how these factors contribute to postpartum maternal and infant health outcomes. Methods Forty-five lactating mothers, with a mean age of 32.
View Article and Find Full Text PDFCold-Spray Deposition of Antibacterial Molybdenum Coatings on Poly(dimethylsiloxane).
ACS Appl Bio Mater
January 2025
Department of Chemistry and Biotechnology; School of Science, Computing & Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
Despite their widespread utilization in biomedical applications, these synthetic materials can be susceptible to microbial contamination, potentially compromising their functionality and increasing the risk of infection in patients. In this study, molybdenum (Mo), an essential metal in biological systems, was investigated as a Mo-based cold-sprayed coating on poly(dimethylsiloxane) (PDMS) for its potential use as biocompatible and antimicrobial surfaces for biomedical applications. Various cold-spray parameters were employed in the fabrication of Mo-embedded PDMS surfaces to alter the surface structure of the substrate, Mo loading density, and embedding layer thickness.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!