Inferences on the nature of a Cr(V) or Cr(IV) species formed by reduction of dichromate by a bovine liver homogenate: NMR and mass-spectrometric studies. | LitMetric
A low-molecular weight chromium-containing fraction of the material resulting from dichromate reduction by bovine liver homogenate was investigated by NMR and ES-MS. The ES-MS spectrum showed a readily detectable peak at m/z = 786.1. The same molecular weight reasonably agreed with the relatively low diffusion coefficient measured by NMR-DOSY experiments on the main species observed in the (1)H NMR spectrum. At least two downfield shifted and broad paramagnetic signals were apparent in the (1)H NMR spectrum. Temperature dependence of chemical shift was exploited in order to estimate the diamagnetic shift of the signals in the diamagnetic region of the spectrum. 2D TOCSY, NOESY, COSY and (1)H-(3)C HMQC spectra revealed the presence of aromatic protons (which were assigned as His residues), Gly and some other short chain amino-acids. Combinations of the molecular masses of such components together with acetate (which is present in the solution) and chromium atoms allowed a tentative proposal of a model for the compound.
Background: Blastocystis is a prevalent intestinal parasitic protist that infects both birds and animals. There are at least 44 subtypes (ST) of Blastocystis, with ST1-ST9 being found in humans. The correlation between specific subtypes and pathogenicity has not been definitively established.
Resource attractiveness and preference is determinant to assess how biodiversity is structured in different ecosystems. Necrophagy is the alternative or complementary dietary habit of dung beetles, but a few studies have focused on evaluating how different carrion types attract different species. The goal of this study was to assess the effect of carrion type on attractiveness and preference of dung beetle taxonomic diversity in a region of Central Amazon.
Introduction: The gastrointestinal microbiota profoundly influences the health and productivity of animals. This study aimed to characterize microbial community structures of the mouth, gastrointestinal tract (GIT), and feces of cattle.
Methods: Samples were collected from 18 Akaushi crossbred steers at harvest from multiple locations, including the oral cavity, rumen, abomasum, duodenum, jejunum, ileum, cecum, spiral colon, distal colon, and feces.
Fasciolosis is a food-borne anthropozoonotic disease caused by Fasciola spp. that affects multiple hosts, including ruminants and humans. In vitro testing of anthelmintics is of interest to establish the drug's activity without the need for time-consuming and expensive in vivo assays.
Changes in maternal nutrition during the periconceptional period can influence postnatal growth in cattle. This study aimed to identify the impact of supplementing beef cows with rumen-protected methionine (RP-Met) during the periconceptional period on their female progeny. In exp 1, plasma methionine (Met) levels were analyzed in samples from 10 Angus crossbred, non-lactating beef cows.
