Production and characteristics of a bioflocculant produced by Bacillus sp. F19.

Bioresour Technol

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Published: November 2008

AI Article Synopsis

  • A soil-isolated bacterium identified as Bacillus sp. produces a bioflocculant called MBFF19, whose production is influenced by various physico-chemical conditions like pH and nutrient sources.
  • Chemical analysis shows that MBFF19 is a sugar-protein derivative, mainly composed of uronic acid and includes components like mannose and glucose.
  • The flocculating properties of MBFF19 were tested with different materials, revealing that while cation supplements didn't enhance flocculation, the presence of Fe3+ actually inhibited it, indicating that pH and bioflocculant dosage are key factors for effective flocculation.

Article Abstract

A bioflocculant-producing bacterium isolated from soil was identified as Bacillus sp. and the bioflocculant produced was named MBFF19. Effects of physico-chemical conditions including pH, carbon sources and nitrogen sources on MBFF19 production were studied. Chemical analyses of the purified bioflocculant MBFF19 indicated that it was a sugar-protein derivative, composed of neutral sugar (3.6%, w/w), uronic acid (37.0%, w/w), amino sugars (0.5%, w/w) and protein (16.4%, w/w). The two neutral sugar components were mannose and glucose and the molar ratio was 1.2:1. Infrared spectrophotometry analysis revealed that MBFF19 contained carboxyl, hydroxyl and methoxyl groups in its structural. Flocculating properties of bioflocculant MBFF19 was examined using kaolin, activated carbon and fly coal suspension. Cation supplement had no positive effects on the flocculating activity whereas the presence of Fe3+ inhibited flocculation. Influences of pH and bioflocculant dosage on the flocculation were also examined.

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Source
http://dx.doi.org/10.1016/j.biortech.2008.01.068DOI Listing

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