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Retrovirus-derived shuttle vectors in the generation of murine bispecific MoAbs to be used in ex vivo and in vivo immunotherapy. | LitMetric
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Retrovirus-derived shuttle vectors in the generation of murine bispecific MoAbs to be used in ex vivo and in vivo immunotherapy.

L B Demonte P Dellabona P Nisticò R Tecce M Camagna A Reyna M Momo P G Natali M Mariani F Malavasi

Ann Ist Super Sanita

Dipartimento di Genetica, Biologia e Chimica Medica, Università degli Studi, Turin, Italy.

Published: December 1991

Mouse monoclonal antibodies specific for CD3, FcR and a melanoma associated antigen have been produced. Drug resistence of such hibrydomas has been obtained tranfecting them with plasmids containing genes conferring specific resistence. Retrovirus derived shuttle vectors with high transfection efficiency have been used for transfection. Hybrydomas were than fused and bispecific antibody producing cells selected. Purification was performed by HPLC. Such bispecific antibodies can be used in ex vivo and in vivo immunotherapy.

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