We observed that Lactobacillus reuteri JCM1112 produces B(12) and folate. However, the folate/B(12) mass ratio found was far below that desired for human consumption ( approximately 170:1). We used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394963 | PMC |
http://dx.doi.org/10.1128/AEM.02719-07 | DOI Listing |
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