Isolation and purification on human kidney hexosaminidases A and B (EC 3.2.1.30) were carried out. The regulation of the supramolecular organization and catalytic activity of the hexosaminidases in the AOT reversed micellar system modelling the enzyme microenvironment in the lysosomes, were investigated. It was found that under these conditions hexosaminidases A and B associate to form a 280-300 kDa dimeric complex. At pH 4.75 hexosaminidases A and B can be isolated from kidney tissue only within the composition of the complex.
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