Objective: To evaluate duration tuning in the inferior colliculus (IC) of guinea pigs and the role of GABA-mediated inhibition on this tuning.
Methods: Totally 23 healthy albino guinea pigs of either sex were employed in this study. After anesthesia, spikes of neurons in inferior colliculus were recorded using five-barrel glass-pipettes. The characteristic frequency was determined by recording iso-intensity response curves at moderate intensity level (40-70 dB SPL) and duration tuning was measured with signals of fixed intensity and varied durations. GABA-A receptor antagonist, bicuculline, was applied to neurons by means of in vivo micro-iontophoresis through one channel in the five-barrel glass-pipettes.
Results: IC neurons of guinea pigs, especially for those who showed sustained temporal response pattern, showed stronger duration tuning in their transient response peak to signal onset. Among 207 neurons recorded, totally 93 neurons were found to show clear duration selectivity. The duration selectivity was eliminated or turned to be weaker in most of the neurons in which the effect of bicuculline was observed successfully.
Conclusions: Unlike what was reported in bats, duration selectivity may be a transient process for most of IC neurons in guinea pigs. Duration selectively of IC neurons in the guinea pig was also largely dependent on the GABAergic inhibition.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effects of cytochalasin D on relaxation process of skinned taenia cecum and carotid artery from guinea pig.
J Physiol Sci
January 2025
Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashiogu, Arakawa-Ku, 116-8551, Tokyo, Japan. Electronic address:
Actin linked regulatory mechanisms are known to contribute contraction/relaxation in smooth muscle. In order to clarify whether modulation of polymerization/depolymerization of actin filaments affects relaxation process, we examined the effects of cytochalasin D on relaxation process by Ca removal after Ca-induced contraction of β-escin skinned (cell membrane permeabilized) taenia cecum and carotid artery preparations from guinea pigs. Cytochalasin D, an inhibitor of actin polymerization, significantly suppressed the force during relaxation both in skinned taenia cecum and carotid artery.
View Article and Find Full Text PDFItaconate mechanism of action and dissimilation in .
Proc Natl Acad Sci U S A
January 2025
Centre for Tuberculosis Research, Tuberculosis Research Laboratory, Translational Health Science and Technology Institute, National Capital Region Biotech Science Cluster 3rd Milestone, Faridabad, Haryana 121001, India.
Itaconate, an abundant metabolite produced by macrophages upon interferon-γ stimulation, possesses both antibacterial and immunomodulatory properties. Despite its crucial role in immunity and antimicrobial control, its mechanism of action and dissimilation are poorly understood. Here, we demonstrate that infection of mice with increases itaconate levels in lung tissues.
View Article and Find Full Text PDFPhosphate rebinding induces force reversal via slow backward cycling of cross-bridges.
Front Physiol
January 2025
Institute of Vegetative Physiology, University of Cologne, Köln, Germany.
Objective: Previous studies on muscle fibers, myofibrils, and myosin revealed that the release of inorganic phosphate (P) and the force-generating step(s) are reversible, with cross-bridges also cycling backward through these steps by reversing force-generating steps and rebinding P. The aim was to explore the significance of force redevelopment kinetics (rate constant ) in cardiac myofibrils for the coupling between the P binding induced force reversal and the rate-limiting transition for backward cycling of cross-bridges from force-generating to non-force-generating states.
Methods: and force generation of cardiac myofibrils from guinea pigs were investigated at 0.
Microneedle-mediated intradermal delivery of Bacille Calmette-Guérin (BCG) vaccines for single-dose tuberculosis vaccination.
Tuberculosis (Edinb)
January 2025
Clinical Research Center, Masan National Tuberculosis Hospital, Changwon, 51755, Republic of Korea. Electronic address:
Tuberculosis (TB) remains a highly lethal infectious disease. The primary preventive measure is Bacille Calmette-Guérin (BCG), a live attenuated vaccine. However, the current intradermal vaccination method with 10-dose vials faces challenges such as inadequate infant injection, inaccurate dispensing, and unstable storage.
View Article and Find Full Text PDFExpression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection.
J Immunol Methods
January 2025
ICAR-Indian Veterinary Research Institute, Bangalore, Karnataka 560024, India.
Bluetongue (BT) is a vector-borne viral disease of multiple domestic and wild ruminants across the globe. The VP7 protein of bluetongue virus (BTV) is the major immune-dominant structural protein that is conserved across the BTV serotypes and therefore, targeted for the development of immuno-diagnostics for BT. In this study, full-length recombinant VP7 protein (rVP7) of BTV-1 was expressed in Trochoplusia ni derived insect cells (Tn5) using codon-optimized synthetic gene construct through baculovirus expression system.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!