Bone mineralization is a result of the function of bone-forming osteoblasts. Osteoblast differentiation from their precursors is a carefully controlled process that is affected by many signaling molecules. Protein-rich food-derived bioactive peptides are reported to express a variety of functions in vivo. We studied the long-term in vitro effect of bioactive tripeptide Ile-Pro-Pro (IPP) on osteoblasts differentiated from human mesenchymal stem cells. Osteoblast bone alkaline phosphatase activity (bALP), bone-forming capacity and gene expression were investigated. Treatment with 50 microM IPP had no effect on bALP activity, but osteoblast mineralization was increased. Gene expression of beta-catenin, Cbfa1/Runx2, PTHrP, CREB-5, osteoglycin, osteocalcin, caspase-8, osteoprotegerin (OPG) and RANKL was analyzed by quantitative real-time PCR on Days 13, 17 and 20 of culture. The results indicate that IPP increased mineral formation due to enhanced cell survival and matrix formation. In addition, IPP reduced the RANKL/OPG ratio. Bioactive peptides, such as IPP, could be one method by which a protein-rich diet promotes bone integrity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jnutbio.2007.09.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ile-Pro-Pro attenuates sympathetic activity and hypertension.
J Physiol Biochem
August 2024
Emergency Department, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210011, China.
Article Synopsis
- Isoleucine-proline-proline (IPP) is a natural tripeptide that inhibits angiotensin-converting enzyme (ACE), playing a role in reducing hypertension and sympathetic activity in a rat study.
- The study involved male Sprague-Dawley rats with induced hypertension, showing that IPP injections into the brain and bloodstream effectively lowered both sympathetic nerve activity and blood pressure by mitigating ACE activity and oxidative stress.
- Long-term treatment with IPP also led to significant reductions in hypertension and vascular changes, highlighting its potential as a therapeutic approach for managing high blood pressure.
Molecular Targets and Mechanisms of Casein-Derived Tripeptides Ile-Pro-Pro and Val-Pro-Pro on Hepatic Glucose Metabolism.
J Agric Food Chem
December 2023
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
The objective of this study was to explore the molecular targets and mechanism of Ile-Pro-Pro (IPP) and Val-Pro-Pro (VPP) on regulating glucose metabolism in hepatic cells and their in vivo hypoglycemic activities in mice. Results showed that both IPP and VPP (600 μM) significantly enhanced the glucose consumption in HepG2 cells and primary hepatocytes ( < 0.05).
View Article and Find Full Text PDFFormulation, Characterisation and Evaluation of the Antihypertensive Peptides, Isoleucine-Proline-Proline and Leucine-Lysine-Proline in Chitosan Nanoparticles Coated with Zein for Oral Drug Delivery.
Int J Mol Sci
September 2022
UCD School of Veterinary Medicine, UCD Conway Institute, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland.
Isoleucine-Proline-Proline (IPP) and Leucine-Lysine-Proline (LKP) are food-derived tripeptides whose antihypertensive functions have been demonstrated in hypertensive rat models. However, peptides display low oral bioavailability due to poor intestinal epithelial permeability and instability. IPP and LKP were formulated into nanoparticles (NP) using chitosan (CL113) via ionotropic gelation and then coated with zein.
View Article and Find Full Text PDFPeptide Analogues of VPP and IPP with Improved Glucose Uptake Activity in L6 Myotubes can be Released from Cereal Proteins.
J Agric Food Chem
March 2021
Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Ag/For Building, Edmonton, Alberta T6G 2P5, Canada.
VPP (Val-Pro-Pro) and IPP (Ile-Pro-Pro) are two famous antihypertensive peptides with possible benefits for type 2 diabetes mellitus (T2DM). The study was aimed to investigate the effect of peptide analogues of VPP and IPP on glucose uptake activity in L6 myotubes. The analogues were designed by replacing the N-terminal, middle, or C-terminal amino acid residues of VPP and IPP with one amino acid at a time from five amino acid groups (polar, nonpolar, basic, acidic, and aromatic amino acids).
View Article and Find Full Text PDFThe Protective Effects of Tripeptides VPP and IPP against Small Extracellular Vesicles from Angiotensin II-Induced Vascular Smooth Muscle Cells Mediating Endothelial Dysfunction in Human Umbilical Vein Endothelial Cells.
J Agric Food Chem
November 2020
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, P.R. China.
Endothelial dysfunction is a common disorder of vascular homeostasis in hypertension characterized by oxidative stress, malignant migration, inflammatory response, and active adhesion response of endothelial cells. The extracellular vesicles (EVs), a vital participant in vascular cell communication, have been considered responsible for vascular disease progression. However, the potential mechanism of antihypertensive peptides against the EVs-induced endothelial dysfunction is still unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!