Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
For this study, 68 plum samples were collected from 12 provinces of China. Low molecular weight RNAs were extracted and used for dot-blot, reverse transcription-polymerase chain reaction (RT-PCR), return-polyacrylamide gel electrophoresis, and biological indexing using cucumber. Results showed that 15 out of the 68 plum samples were positive for Hop stunt viroid (HSVd). Four positive samples were selected for cloning and sequence analysis. Results indicated that most HSVd sequences from the plum in China had 1-3 nucleotide changes from the closest HSVd in GenBank. In addition, the sample PB21 (cv. 'Friar') collected from Hebei province had one sequence with a 15-nt duplication (named HSVd D-15) at the 244/245 position in the lower central region. By biological indexing, cucumber seedlings, an indicator plant for HSVd, were inoculated with RNAs directly extracted from the original plum source (PB21, cv. 'Friar'). Nucleotide sequencing analysis of the progeny showed that HSVd, but not HSVd-D15, was recovered from inoculated cucumbers. Although very unlikely, the possibility that this extra sequence was the result of a PCR artifact cannot be completely ruled out.
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Source |
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http://dx.doi.org/10.1007/s10528-007-9134-6 | DOI Listing |
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