Desorption electrospray ionization (DESI) allows the direct analysis of ordinary objects or pre-processed samples under ambient conditions. Among other applications, DESI is used to identify and record spatial distributions of lipids and drug molecules in biological tissue sections. This technique does not require sample preparation other than production of microtome tissue slices and does not involve the use of ionization matrices. This greatly simplifies the procedure and prevents the redistribution of analytes during matrix deposition. Images are obtained by continuously moving the sample relative to the DESI sprayer and the inlet of the mass spectrometer. The timing of the protocol depends on the size of the surface to be analyzed and on the desired resolution. Analysis of organ tissue slices at 250 microm resolution typically takes between 30 min and 2 h.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/nprot.2008.11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chemical characterization and classification of vegetable oils using DESI-MS coupled with a neural network.
Food Chem
December 2024
Panvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. Electronic address:
This study tackled mislabeling fraud in vegetable oils, driven by price disparities and profit motives, by developing an approach combining desorption electrospray ionization mass spectrometry (DESI-MS) with a shallow convolutional neural network (SCNN). The method was designed to characterize lipids and distinguish between nine vegetable oils: corn, soybean, peanut, sesame, rice bran, sunflower, camellia, olive, and walnut oils. The optimized DESI-MS method enhanced the ionization of non-polar glycerides and detected ion adducts like [TG + Na], [TG + NH].
View Article and Find Full Text PDFMass Spectrometry Imaging Reveals Spatial Metabolic Alterations and Salidroside's Effects in Diabetic Encephalopathy.
Metabolites
December 2024
Key Laboratory of Mass Spectrometry Imaging and Metabolomics, Minzu University of China, National Ethnic Affairs Commission, Beijing 100081, China.
Diabetic encephalopathy (DE) is a neurological complication of diabetes marked by cognitive decline and complex metabolic disturbances. Salidroside (SAL), a natural compound with antioxidant and neuroprotective properties, has shown promise in alleviating diabetic complications. Exploring the spatial metabolic reprogramming in DE and elucidating SAL's metabolic effects are critical for deepening our understanding of its pathogenesis and developing effective therapeutic strategies.
View Article and Find Full Text PDFSpatial Distribution and Comparative Analysis of Differential Metabolites in Curcuma longa L. Roots and Rhizomes Using UHPLC-Q-Orbitrap HRMS Combined With DESI-MSI.
Phytochem Anal
December 2024
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Introduction: The roots and rhizomes of Curcuma longa L. serve as distinct traditional Chinese medicines with varying therapeutic effects, likely attributed to differences in the accumulation and distribution of metabolites in these parts.
Objective: The study aims to investigate the differences and spatial distribution patterns of metabolites in C.
In the realm of document examination, the identification of suspicious alterations to handwritten documents is an important factor in case characterization. Investigating the differences in gel pen ink compositions has significant implications. In this study, we used desorption electrospray ionization mass spectrometry (DESI-MS) to analyze the ink compositions of gel pens.
View Article and Find Full Text PDF[Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization].
Se Pu
January 2025
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Ambient mass spectrometry imaging (MSI) enables hundreds of analytes in tissue sections to be directly mapped at atmospheric pressure with minimal sample preparation. This field is currently experiencing rapid growth, with numerous reported ambient ionization techniques resulting in a "hundred flowers bloom" situation. Nanospray desorption electrospray ionization (nano-DESI), developed by the Laskin group in 2010, is a widely used liquid-extraction-based ambient ionization technique that was first used for mass spectrometry imaging of tissue in 2012.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!