A simple, reliable and accurate method for the simultaneous separation and determination of 10 active components (psoralen, isopsoralen, emodin, oleanolic acid, stachydrine hydrochloride, ammonium glycyrrhizinate, glycyrrhizinate, schizandrol, imperatorin and isoimperatorin) in traditional Chinese medicine "YIGONG" capsule was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with diode array detection. The chromatographic separation was performed on a Eurospher C(18) column (250 mm x 4.6 mm i.d. with 5.0 microm particle size) with a acetonitrile-water gradient containing 0.5% (v/v) aqueous phosphoric acid as mobile phase. Two detection wavelengths (210 and 250 nm) were utilized for the quantitative analysis due to the different UV spectra of these components. Good linear behaviors over the investigated concentration ranges were observed with the values of R(2) higher than 0.999 for all the analytes. The recoveries, measured at three concentration levels, varied from 95.0 to 105.3%. The validated method was successfully applied to the simultaneously determination of these active components in "YIGONG" capsule from different production batches.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jpba.2008.01.019 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intravenous administration of mitochondria improves ovarian function by anti-apoptosis in the premature ovarian insufficiency model.
Climacteric
January 2025
Department of Gynecology and Obstetrics, Guizhou Provincial People's Hospital, Guiyang, China.
Objective: For patients with contraindications to hormone therapy, the absence of effective treatments for ovarian dysfunction post chemotherapy represents a critical issue requiring resolution. Local administration of mitochondria may enhance ovarian function in premature ovarian insufficiency (POI) by ameliorating diminished mitochondrial activity. Nevertheless, there is a paucity of literature on the efficacy of mitochondrial transplantation through intravenous injection, a less invasive and more convenient method than local injection, for the improvement of ovarian function in POI following chemotherapy.
View Article and Find Full Text PDFIntegrated UHPLC-mS/mS analysis, spectrum-effect relationship, and molecular docking to study the antidiabetic components of .
Nat Prod Res
January 2025
Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, PR China.
The leaves of (Batal) Iljinsk., a plant native to China that has long been used in traditional Chinese medicine to treat diabetes. It remains to be determined what chemical constituents are responsible for this effect.
View Article and Find Full Text PDFClassifying Impact Loading Using Axial Peak Tibial Acceleration and Impact-Related Biomechanical Differences During Treadmill Running.
Scand J Med Sci Sports
January 2025
Faculty of Medicine, Health, and Human Sciences, Macquarie University, Sydney, New South Wales, Australia.
Measuring lower extremity impact acceleration is a common strategy to identify runners with increased injury risk. However, existing axial peak tibial acceleration (PTA) thresholds for determining high-impact runners typically rely on small samples or fixed running speeds. This study aimed to describe the distribution of axial PTA among runners at their preferred running speed, determine an appropriate adjustment for investigating impact magnitude at different speeds, and compare biomechanics between runners classified by impact magnitude.
View Article and Find Full Text PDFCharacterizing astrocyte-mediated neurovascular coupling by combining optogenetics and biophysical modeling.
J Cereb Blood Flow Metab
January 2025
Neuronal Mass Dynamics Lab, Department of Biomedical Engineering, Florida International, University, Miami, FL, USA.
Vasoactive signaling from astrocytes is an important contributor to the neurovascular coupling (NVC), which aims at providing energy to neurons during brain activation by increasing blood perfusion in the surrounding vasculature. Pharmacological manipulations have been previously combined with experimental techniques (e.g.
View Article and Find Full Text PDFGenotypic diversity and biological characterization of Alphabaculovirus mabrassicae isolates from a wide host range.
Pest Manag Sci
January 2025
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Background: Baculoviruses are ideal biological insecticides, providing long-lasting pest control and environmental benefits. Alphabaculovirus mabrassicae stains, with their broad host range, have been effective in agricultural pest management. Various A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!