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Mutation of cysteine residues alters the heme-binding pocket of indoleamine 2,3-dioxygenase-1.
Biochem Biophys Res Commun
July 2013
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
The hemoprotein indoleamine 2,3-dioxygenase-1 (IDO1) is the first and rate-limiting enzyme in mammalian tryptophan metabolism. Interest in IDO1 continues to grow, due to the ever expanding influence IDO1 plays in the immune response. This study examined the contribution of all individual cysteine residues towards the overall catalytic properties and stability of recombinant human IDO1 via mutagenesis studies using a range of biochemical and spectroscopic techniques, including in vitro kinetic assessment, secondary structure identification via circular dichroism spectroscopy and thermal stability assessment.
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