The second start-up of anaerobic ammonium oxidation (ANAMMOX) process with landfill leachate was investigated. It works after the ANAMMOX bacteria in the UASB-ANAMMOX reactor have been fully restrained by temperature and influent. The results reveal that the second start-up was faster, and the removal of NH4(+) -N and NO2(-) -N was up to 96.17% and 86.77% on the 21th day, respectively. The decrease of the synergetic mechanism of denitrification with ANAMMOX result in a declining trend of the removal of COD and the average quantity of removal was only 60 mg/L. The average ratio of removed ammonia , removed nitrite and produced nitrate was 1:0.75 : 0.26 in the second start-up process, and it was 1:0.95 : 0.26 at the stable stage of second start-up of ANAMMOX process. The removal of nitrite in the Three-Nitrogen-Compounds-Ratio has raised significantly.
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