AI Article Synopsis
- The human RNA-binding protein La is crucial for poliovirus translation but suppresses translation and replication of hepatitis A virus (HAV).
- La specifically interacts with different regions of the HAV IRES, differing from its role in poliovirus.
- Unlike poliovirus, HAV's proteinase 3C does not cleave La, suggesting distinct mechanisms in how these viruses utilize IRES elements for translation.
Article Abstract
The human RNA-binding protein La, is an essential trans-acting factor in IRES-dependent translation initiation of poliovirus, the prototypic picornavirus. For hepatitis A virus (HAV), an unusual member of this virus family, the role of host proteins in its inefficient translation and slow replication is unclear. Using small interfering RNA in vivo and purified La in vitro, we demonstrate for the first time that La suppresses HAV IRES-mediated translation and replication. We show that La binds specifically to distinct parts of the HAV IRES and that-unlike poliovirus-HAV proteinase 3C does not cleave La. The La-mediated suppression of HAV translation and stimulation of poliovirus translation implies unexpected mechanistic differences between viral IRES elements.
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| http://dx.doi.org/10.1016/j.bbrc.2008.01.163 | DOI Listing |
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