Hydrolases: the correlation between informational structure and the catalytic sites organization.

J Biomol Struct Dyn

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, ul. Miklukho-Maklaya 16/10, Russia.

Published: April 2008

The novel method allowing identification of protein structure elements responsible for catalytic activity manifestation is proposed. Structural organization of various hydrolases was studied using the ANIS (ANalysis of Informational Structure) method. ANIS allows to reveal a hierarchy of the ELements of Information Structure (ELIS) using protein amino acid sequence. The ELIS corresponds to the variable length sites with an increased density of structural information. The amino acid residues forming the enzyme catalytic site were shown to belong to the different top-ranking ELIS located in the contact area of the corresponding spatial structure clusters. In the protein spatial structure catalytic sites are located in the area of contact between fragments of polypeptide chain (structural blocs) allocation to the different top-ranking ELIS. According to our results we concluded that structural blocks corresponding to top-ranking ELIS are crucial for protein functioning. Such regions are structurally independent, and their determinate mobility relative to each other is vital for an efficient enzymatic reaction to occur.

Download full-text PDF

Source
http://dx.doi.org/10.1080/07391102.2008.10507202DOI Listing

Publication Analysis

Top Keywords

top-ranking elis
12
informational structure
8
structure catalytic
8
catalytic sites
8
amino acid
8
spatial structure
8
structure
6
elis
5
hydrolases correlation
4
correlation informational
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!