AI Article Synopsis
- The study aimed to observe the immune response and healing process of porcine cancellous bone scaffolds (PCBS) after they are implanted in rabbits.
- The research involved implanting PCBS and measuring tissue response and cytokine expression at various time points post-surgery to analyze inflammation and immune reactions.
- Results showed an initial inflammatory response dominated by Th1 cytokines that declined over time, while Th2 cytokine levels increased and peaked at 8 weeks, suggesting a shift from inflammation to healing in response to the scaffold.
Article Abstract
Objective: To observe the dynamic immunity of the porcine cancellous bone scaffold(PCBS) after transplantation and provide experimental evidences for amending and clinical use.
Methods: The PCBS was gained through physical and chemical disposals to porcine cancellous bone and was implanted subcutaneously in rabbit. The histological investigation and the expression of Th1 (such as IL-2, IFN-gamma) and Th2 (such as IL-4, IL-10) mRNA were measured by HE, RT-PCR respectively in 1,2,4,8,12 week postoperation.
Results: HE revealed: Inflammation cells (lymphocytes and monocytes) were shown abundantly in the surrounding tissue in 1 week and were decreased gradually since 2 week. At 12 week, there were few inflammation cells in the surrounding tissue. RT-PCR revealed: The mRNA expression level of Th1 cytokines such as IL-2, IFN-gamma was high in 1 week and began to fall since 2 week. At 12 week, the mRNA expression level of Th1 cytokines was low; The mRNA expression level of Th2 cytokines such as IL-4, IL-10 was low in 1 week, rose gradually since 2 week and reached tiptop in 8 week. At 12 week, the mRNA of Th2 cytokines maintained high level expression.
Conclusions: The porcine cancellous bone scaffold excitate immunoreaction was transitory inflammation after implantation.
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