Identification of RNA binding proteins by UV cross-linking.

Curr Protoc Mol Biol

Harvard Medical School, Boston, Massachusetts, USA.

Published: August 2003

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Article Abstract

One of the major focuses of modern biology is to understand the dynamics of RNA-protein interactions, including factors that interact with mRNA, rRNA, tRNA, snRNA, hnRNA, siRNA, and viral RNA. Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this endeavor.

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http://dx.doi.org/10.1002/0471142727.mb2702s63DOI Listing

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