Importance of intermediary transitions and waveform in the enzyme-electric field interaction.

The current theory of enzymes and electric field interaction does not account for all the observed data since we could not observe non-linear behavior of cell suspensions as anticipated by other authors. In our case, we used a pure sinusoidal source, however the experiments that do account for responses used a sum of a central sinusoidal and its harmonics frequencies. As a result, we suggest that the enzyme and electric interaction are favored when the field has a non-strictly sinusoidal waveform, and this behavior is related to the true intermediate transitions of the enzyme during its catalytic cycle. Therefore, we developed an iterative model of the interaction process based on previous models and actual trends. The model well verified all the previous simulations and showed that, for a non-symmetrical enzyme, the energy can harvest its maximal for non sinusoidal fields.