[Endogenic intoxication and free radical oxidation in urolithiasis].

The aim of the study was elucidation of relations between endogenic intoxication (EI) and free radical oxidation (FRO) in patients with urolithiasis and influence of EI and FRO on development of urolithiasis. The study included 35 urolithiasis patients (the study group) and 35 healthy controls aged 30 to 72 years. EI was assessed by content of middle molecular peptides (MMP) in the blood serum and quality-quantity composition of organic substances adsorbed on the surface of erythrocytic membrane. Investigation of adsorbed organic substances on erythrocytic membrane was conducted by assessment of adsorption-transport function of erythrocytes (ATFE) defined by means of erythrocytic wash-outs (EW) obtained according to R. A. Gareev technique (1998). Data processing was made with statistic package Microsoft Excel-2000 in S. N. Lapach modification (2000). A comparative level of MMP washed from erythrocytic membranes in urolithiasis patients is significantly elevated (p < 0.002). Total content of organic substances in the blood of urolithiasis patients is significantly increased (p < 0.01) due to their erythrocytic component. The study group had enhanced FRO processes (p < 0.01). A significant direct correlation was found between LPO product and EW, MMP (r = from +0.42 to +0.61; p < 0.05), inverse correlation between lipid peroxidation (LPO) and EW, MMP (r = from -0.54 to -0.62; p < 0.05). Adirect correlation exists between MMP level and EW (r = from +0.82 to +0.92, p < 0.01). Thus, urolithiasis patients have significant enhancement of LPO and EI. LPO activity in the blood of urolithiasis patients correlates with EI.