Objective: It is unknown which genes are differentially expressed in cultured epithelial cells derived from the epithelial rests of Malassez (ERM) in periodontal ligament and oral gingival epithelium (OGE). This study analysed the different gene expression of OGE and ERM cells using a DNA microarray technique.
Design: Epithelial cells from ERM and OGE were isolated from porcine periodontal ligament and oral gingival epithelium. Each RNA sample extracted from the cells was reverse transcribed into cDNA and labelled with either cytidine 5-dUTP (Cy5) or cytidine 3-dUTP (Cy3). These labelled cDNA probes were then mixed and simultaneously hybridised to the Pig 13K microarray plate bearing 13,295 different genes (Operon, AL). Cellular enzyme-linked immunosorbent assay (CELISA) was performed to confirm the expression at protein level.
Results: There were nine genes common to the triplicate microarrays in ERM cells and one in OGE cells. Four of the nine genes including tissue factor (TF), FAT cadherin (FAT) and two unknown genes were expressed at levels more than threefold higher in ERM cells than in OGE cells. The protein levels of both TF and FAT in ERM cells were significantly higher than those in OGE.
Conclusion: TF and FAT may act as markers to distinguish ERM cells from OGE cells in vitro.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.archoralbio.2007.12.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In Vitro Effects of Recombinant Human Neuritin on Hair Cell Recovery Post-Gentamicin Injury in SC Lineage-Tracing Models: Involvement of Notch and FGFR Signaling.
Neurochem Int
January 2025
School of Public Health, Hangzhou Normal University, Hangzhou, 311121, P. R. China. Electronic address:
Hair cell (HC) loss, frequently induced by ototoxic agents such as gentamicin, leads to irreversible hearing loss. Because of the restricted regenerative capabilities of the mammalian inner ear, the exploration of therapeutic strategies to restore damaged HCs is critically needed. Recombinant human Neuritin (rhNeuritin), a neurotrophic factor with established roles in promoting cell survival and regeneration across various systems, presents itself as a promising therapeutic candidate for HC repair.
View Article and Find Full Text PDFCells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the polarized bleb morphology of metastatic melanoma cells migrating in non-adhesive confinement. Using high-resolution time-lapse imaging and specific molecular perturbations, we found that EGF signaling via PI3K stabilizes and maintains a polarized leader bleb.
View Article and Find Full Text PDFMicrocystoid macular edema in epiretinal membrane: not a retrograde maculopathy.
Am J Ophthalmol
January 2025
Department of biomedical sciences, Humanitas University, Pieve Emanuele, Milan, Italy.; Ophthalmology Department, Humanitas Gavazzeni, Bergamo, Italy.
Purpose: To investigate the incidence, clinical spectrum and pathophysiology of microcystoid macular edema (MME) in two cohorts of patients with epiretinal membrane (ERM) and idiopathic full thickness macular hole (FTMH).
Design: Single-center, Retrospective, interventional, cohort study.
Methods: Review of clinical charts, structural and en-face optical coherence tomographty (OCT) and fluorescein angiography (FA) imaging of ERM and FTMH eyes which underwent surgery with pars plana vitrectomy and internal limiting membrane (ILM) peel, with a minimum follow-up of 6 months.
TNIK: A redox sensor in endothelial cell permeability.
Sci Adv
December 2024
School of Cardiovascular and Metabolic Medicine and Sciences, James Black Centre, BHF Centre of Research Excellence, 125 Coldharbour Lane, King's College London, London SE5 9NU, UK.
Dysregulation of endothelial barrier integrity can lead to vascular leak and potentially fatal oedema. TNF-α controls endothelial permeability during inflammation and requires the actin organizing Ezrin-Radixin-Moesin (ERM) proteins. We identified TRAF2 and NCK-interacting kinase (TNIK) as a kinase directly phosphorylating and activating ERM, specifically at the plasma membrane of primary human endothelial cells.
View Article and Find Full Text PDFVitreous Cytokine Profile in an Eye with a Vasoproliferative Tumor.
Retin Cases Brief Rep
December 2024
Department of Ophthalmology, NYU Langone Health, New York, NY.
Purpose: We present a patient with a primary vasoproliferative tumor (VPT) accompanied by vitreous haze and an epiretinal membrane (ERM). We report for the first time the vitreous cytokine profile from an eye with a primary VPT to explore the relationship between intraocular inflammation and these tumors.
Methods: Retrospective chart review of a single patient case.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!