Prooxidant and antioxidant properties of nitric oxide (NO) during oxidative stress are mostly dependent on its interaction with reactive oxygen species, Fe ions, and hemoproteins. One form of NO storage and transportation in cells and tissues is dinitrosyl iron complexes (DNIC), which can bind with both low-molecular-weight thiols and proteins, including hemoglobin. It was shown that dinitrosyl iron complexes bound with hemoglobin (Hb-DNIC) were formed in rabbit erythrocytes after bringing low-molecular-weight DNIC with thiosulfate into blood. It was ascertained that Hb-DNIC intercepted free radicals reacting with hemoglobin SH-groups and prevented oxidative modification of this protein caused by hydrogen peroxide. Destruction of Hb-DNIC can take place in the presence of both hydrogen peroxide and tert-butyl hydroperoxide. Hb-DNIC can also be destroyed at the enzymatic generation of superoxide-anion radical in the xanthine-xanthine oxidase system. If aeration in this system was absent, formation of the nitrosyl R-form of hemoglobin could be seen during the process of Hb-DNIC destruction. Study of Hb-DNIC interaction with reactive oxygen metabolites is important for understanding NO and Hb roles in pathological processes that could result from oxidative stress.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S0076-6879(08)36025-X | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reduction of Nitrite in an Iron(II)-Nitrito Compound by Thiols and Selenol Produces Dinitrosyl Iron Complexes via an {FeNO} Intermediate.
Inorg Chem
December 2024
School of Chemical Sciences, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India.
Reaction of an Fe(II) complex, [Fe(6-COO-tpa)] (), with PhE and NO produced [Fe(6-COO-tpa)(EPh)] (E = S, ; Se, ) and [Fe(6-COO-tpa)κO,O'-NO)] (), respectively (6-COOH-tpa is bis(2-pyridylmethyl)(6-carboxyl-2-pyridylmethyl)amine). Treatment of with 2 equiv of PhEH (E = S, Se) produced NO in ∼40% yields, respectively, along with and the DNICs, [Fe(EPh)(NO)] (E = S, Se). Treatment of with excess PhEH produced NO in similar yields, while was converted to the same DNICs and / (instead of ).
View Article and Find Full Text PDFNitrogen Oxide (NO) in the Pathogenesis of Preeclampsia.
Bull Exp Biol Med
October 2024
Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
The concentrations of nitric oxide (NO) donors in the plasma of pregnant women with preeclampsia is several times higher than in healthy pregnant women. Antihypertensive drugs acting not through the NO-mediated mechanisms normalized BP in some women with preeclampsia, but did not significantly reduce the levels of NO donors in the plasma. It appears that preeclampsia is associated with insufficient NO availability for the targets, rather than low intensity of NO synthesis.
View Article and Find Full Text PDFStructure, properties, and decomposition in biological systems of a new nitrosyl iron complex with 2-methoxythiophenolyls, promising for the treatment of cardiovascular diseases.
J Inorg Biochem
January 2025
Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Moscow region, prosp. Akad. Semenova, 1, 142432 Chernogolovka, Russian Federation; Faculty of Fundamental Physical and Chemical Engineering, Lomonosov Moscow State University M.V. Lomonosov, Leninskie gory, 1, 119991 Moscow, Russian Federation; Scientific and Educational Center "Medical Chemistry" in Chernogolovka, Federal State Autonomous Educational Institution of Higher Education "State University of Education", Moscow Region, st. Vera Voloshina, 24, 141014 Mytishchi, Russian Federation.
A new promising binuclear tetranitrosyl iron complex with 2-methoxythiophenolyl of the composition [Fe(CHOS)(NO)] (complex 1), which acts on the therapeutic targets of cardiovascular diseases, guanylate and adenylate cyclase, has been synthesized. X-ray diffraction data show the presence of two isoforms of complex 1; according to quantum chemical calculations, the structure of only the trans isomer is stable in solutions. The processes of transformation of complex 1 in DMSO, in aqueous solutions, as well as in the presence of bovine serum albumin, reduced glutathione, and mucin were studied.
View Article and Find Full Text PDFEffect of Nitrosyl Iron Complex with 3,4-Dichlorothiophenolyls on the Level of Cyclic Nucleotide In Vitro.
Bull Exp Biol Med
June 2024
Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Russia.
The effect of a promising NO donor, a binuclear nitrosyl iron complex (NIC) with 3,4-dichlorothiophenolyls [Fe(SCHCl)(NO)], on the adenylate cyclase and soluble guanylate cyclase enzymatic systems was studied. In in vitro experiments, this complex increased the concentration of important secondary messengers, such as cAMP and cGMP. An increase of their level by 2.
View Article and Find Full Text PDFEffects of Nitrosyl Iron Complexes with Thiol, Phosphate, and Thiosulfate Ligands on Hemoglobin.
Int J Mol Sci
June 2024
Bach Institute of Biochemistry, Federal Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia.
Nitrosyl iron complexes are remarkably multifactorial pharmacological agents. These compounds have been proven to be particularly effective in treating cardiovascular and oncological diseases. We evaluated and compared the antioxidant activity of tetranitrosyl iron complexes (TNICs) with thiosulfate ligands and dinitrosyl iron complexes (DNICs) with glutathione (DNIC-GS) or phosphate (DNIC-PO) ligands in hemoglobin-containing systems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!