AI Article Synopsis
- Scientists used a special analysis called NanoLC-MS/MS to study a protein called CDC25B3 in human cells and see how it gets changed by adding phosphate groups (phosphorylation).
- They found 18 spots on the protein that can be phosphorylated, and most of these were already known (like S/T P), but they also found 8 new spots that were not the usual type.
- They discovered that up to 13 phosphate groups can attach to one CDC25B3 molecule, and many of the phosphorylation sites they found were new and not seen in similar proteins before.
Article Abstract
NanoLC-MS/MS analysis was used to characterize the phosphorylation pattern in vivo of CDC25B3 (phosphatase splice variant 1) expressed in a human cell line and to compare it to the phosphorylation of CDC25B3 by Cdk1/cyclin B and Chk1 in vitro. Cellular CDC25B3 was purified from U2OS cells conditionally overexpressing the phosphatase. Eighteen sites were detectably phosphorylated in vivo. Nearly all existing (S/T)P sites were phosphorylated in vivo and in vitro. Eight non(S/T)P sites were phosphorylated in vivo. All these sites could be phosphorylated by kinase Chk1, which phosphorylated a total of 11 sites in vitro, with consensus sequence (R/K) X(2-3) (S/P)-non P. Nearly half of the sites identified in this study were not previously described and were not homologous to sites reported to be phosphorylated in other CDC25 species. We also show that in vivo a significant part of CDC25B molecules can be hyperphosphorylated, with up to 13 phosphates per phosphatase molecule.
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| http://dx.doi.org/10.1021/pr700623p | DOI Listing |
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