Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Objective: To evaluate effects of centipede on life span of drosophila and on the appearance of the filial generation.
Methods: Drosophila melanogaster (male and female) were cultured in tubes with centipede extraction at concentrations of 0, 0.2%, 1%, 2.5%, or 5% until all drosophila died. The appearance of the filial generation were observed.
Results: The life span, including average, longest and shortest life span was shortened in exposed groups of both sexes and significantly dose-dependent. The appearance of the filial generation showed normal.
Conclusion: Centipede extracts accelerates the aging of drosophila and shortens their life span. The appearance of the filial generation is not affected. Clinic using centipede for a long period should be carefully.
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