AI Article Synopsis
- The text outlines a recommended strategy for researchers on how to choose a method for producing recombinant proteins, drawing from the analysis of over 10,000 proteins.
- It provides a prioritized list of initial approaches and alternative strategies, aimed at helping researchers streamline their decision-making process.
- Additionally, the review identifies common pitfalls that can hinder new investigators in their protein expression and purification efforts.
Article Abstract
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178102 | PMC |
| http://dx.doi.org/10.1038/nmeth.f.202 | DOI Listing |
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