AI Article Synopsis
- A duplex reverse transcriptase polymerase chain reaction-restriction fragment length polymorphism method was performed on 412 patient samples to subtype influenza viruses.
- The assay effectively identified and distinguished between all 47 strains of influenza A H1N1 and H3N2.
- This rapid testing technique can determine if positive samples are current circulating strains or new variants, making it useful as an initial test during prepandemic stages.
Article Abstract
A duplex reverse transcriptase polymerase chain reaction-restriction fragment length polymorphism for influenza virus subtyping was applied to 412 patient samples. The assay was able to discriminate all 47 influenza A H1N1 and H3N2 viruses. This rapid technique assessed if positive samples were current circulating strains or an emergent one and could be used as the 1st test in prepandemic stages.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2007.11.012 | DOI Listing |
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