AI Article Synopsis
- Immunohistochemistry is a critical technique for identifying protein locations in cells and tissues, utilizing both fluorescence and bright-field microscopy.
- This unit covers methods for labeling proteins in both suspended and adherent cultures, as well as in tissue sections.
- It includes guidance on selecting antibodies and detection methods, along with troubleshooting tips to resolve common issues.
Article Abstract
Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.
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| http://dx.doi.org/10.1002/0471142727.mb1406s81 | DOI Listing |
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