Metabolic labeling of glycoproteins with radioactive sugars.

Curr Protoc Cell Biol

Case Western Reserve University, Cleveland, Ohio, USA.

Published: February 2002

This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. Methods for liberation of both N- and O-linked glycans are also described. These protocols can be used to generate materials for characterization of glycoprotein glycans from cultured cells.

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http://dx.doi.org/10.1002/0471143030.cb0708s13DOI Listing

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