Gelled substrates can be used for a variety of in vitro and in vivo experiments. A type I collagen gelled substrate will promote cell growth and differentiation. Gelled Matrigel substrate promotes the survival of explanted cells and tissues and the differentiation of a variety of epithelial and endothelial cell types in vitro and to assess angiogenesis and increase tumor growth in vivo. Preparation of the matrices and their use are described in this unit.
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Centro de Investigación en Alimentación y Desarrollo (CIAD), Coordinación de Tecnología de Alimentos de Origen Animal (CTAOA), Carretera Gustavo Enrique Astiazarán Rosas 46, Hermosillo 83304, Mexico.
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Department of Medical Biochemistry, Molecular Biology, and Immunology, School of Medicine, University of Seville, Av. Sanchez Pizjuan s/n, 41009 Seville, Spain; Instituto de Biomedicina de Sevilla, IBiS/Hospital Universitario Virgen del Rocio/CSIC/Universidad de Sevilla, 41013 Seville, Spain.
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