AI Article Synopsis
- The study aimed to investigate how methotrexate (MTX) affects intestinal mucosal permeability in rats and to explore the protective role of curcumin against this damage.
- Four groups of rats were used: a control group, a model group with induced enteritis, and two treatment groups receiving either sulfasalazine or curcumin. Results showed that the model group exhibited significant signs of intestinal damage, while curcumin treatment notably improved the condition.
- The findings suggest that MTX increases intestinal permeability, and curcumin may help protect against this effect by reducing the associated markers of intestinal damage.
Article Abstract
Objective: To observe the changes in intestinal mucosal permeability in rats with methotrexate (MTX)-induced small intestinal damage and investigate the protective effects of curcumin.
Methods: The experiment was carried out using 4 groups of rats, namely the normal control group, enteritis model group, sulfasalazine (SASP) group and curcumin group. With the exception of the rats in the normal control group, all rats were subjected to intraperitoneal MTX injection to induce enteritis and received subsequent daily intragastric administration of SASP (100 mg/kg), curcumin (100 mg/kg), or normal saline for 5 days. The disease activity index (DAI), colonic mucosal damage index (CMDI) and histological score (HS) of the rats were evaluated. The levels of diamine oxidase (DAO) and D-lactate were assessed using spectrophotometric assay, and myeloperoxidase (MPO) activity and intracellular adhesion molecule-1 (ICAM-1) protein expression were measured by biochemical and immunohistochemical methods, respectively.
Results: Compared with the normal control group, the rats in the model group showed significantly increased DAI, CMDI and HS and levels of DAO, D-lactate, ICAM-1 and MPO. Curcumin treatment resulted in significantly decreased DAI, CMDI, HS and lowered activities of D-lactate, ICAM-1 and MPO in comparison with the model group (P<0.01).
Conclusion: MTX induces increased mucosal permeability of the small intestines in rats, and curcumin may offer protective effects against MTX-induced rat enteritis by lowering the intestinal mucosal permeability.
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